Ratlarda optik kiazmaya uygulanan deneysel bası ile oluşan demiyelinizasyon sonrası myricetin'in remiyelinizasyon üzerine etkilerinin araştırılması

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Thesis Type: Expertise In Medicine

Institution Of The Thesis: Kocaeli University, Tıp Fakültesi, Cerrahi Tıp Bilimleri, Turkey

Approval Date: 2023

Thesis Language: Turkish

Student: EREN YILMAZ

Supervisor: İhsan Anık

Open Archive Collection: AVESIS Open Access Collection

Abstract:

INTRODUCTION: The lack of the central nervous system's ability to regenerate and axonal growth has led to research that can reverse this process for many years. The aim of this study is to show the results of the effect of myricetin, a neuroprotective agent, on remyelination processes after demyelination, by placing a balloon catheter that will compress the optic chiasm in rats, such as demyelination caused by lesions that cause compression on the optic nerve, by electron microscopy. MATERIALS AND METHODS: The study was carried out with three experimental and one control groups using forty Wistar Albino rats. The control group was decapitated after deep anesthesia. The rats in the other groups were fixed with a stereotaxy device under anesthesia and a balloon catheter was inserted under the optic chiasm by opening the burhole 6.2 mm in front of the bregma appropriate to the stereotaxy brain atlas coordinates. Group-2 was decapitated on the 10th day. The catheter was removed on the 10th day in Group-3 and Group-4. Following the removal of the catheter in Group-4, İ.P. 25mg/kg myricetin was administered and these 2 groups were decapitated 10 days later. Optic nerves taken from the groups after decapitation were examined under electron microscope. RESULTS: Severe degenerations indicating demyelination, delamination, degeneration, watery degeneration of axons and Wallerian degeneration accompanied by vacuolization and dark axon degeneration were observed in the demyelination and remyelination groups compared to the control. Compared to the demyelination and remyelination groups, the myricetin group showed a decrease in demyelination, delamination and degeneration, and a decrease in vacuolization and dark axon degeneration in the axon and oligodendrocyte cytoplasm. CONCLUSION: Experimental pressure applied to the optic chiasm in rats showed severe degeneration of the nerve under electron microscopy, and moderate degeneration was demonstrated in the group that was administered myricetin intraperitoneally after the pressure effect disappeared. This degeneration-reducing effect of myricetin, a neuroprotective substance, will contribute to future successive studies.