Ratlarda oluşturulan nazal septal perforasyon modelinde mezenkimal kök hücre uygulamasının nazal septumdaki yara iyileşmesi üzerine etkisinin değerlendirilmesi
DEĞER H. M. (Executive), YAZIR Y., HACIOĞLU O., FURAT S. H., ŞİRİN S.
Project Supported by Higher Education Institutions, BAP PhD, 2025 - 2026
- Project Type: Project Supported by Higher Education Institutions
- Support Program: BAP PhD
- Begin Date: June 2025
- End Date: April 2026
Project Abstract
ABSTRACT
OBJECTIVE: This study aimed to evaluate the effects of dental pulp–derived mesenchymal stem cells (DP-MSCs) on nasal septal wound healing and cartilage regeneration, as well as potential dose-dependent responses, in an experimental nasal septal perforation model created in rats.
MATERIALS AND METHODS: Twenty-five male rats were included in the study. One rat was used for pilot testing, and three rats were designated as healthy controls. The remaining 21 rats were randomly assigned to Sham, 5×10⁵ DP-MSC, and 1×10⁶ DP-MSC groups. A full-thickness septal perforation approximately 2 mm in diameter was created in the right nasal cavity using a 14G intravenous cannula positioned approximately 3 mm posterior to the columella. Tissue scaffolds seeded with 5×10⁵ or 1×10⁶ GFP-labeled DP-MSCs were placed into the perforation site. On postoperative day 14, the septum was completely harvested and evaluated macroscopically and histopathologically.
RESULTS: Although no statistically significant difference in perforation size was observed macroscopically, the only complete closure occurred in the 1×10⁶ DP-MSC group. Histopathological analyses showed more favorable healing in MSC-treated groups compared to the sham group across parameters including epithelial regeneration, epithelial degeneration, fibroblast presence, collagen deposition, capillary density, acute inflammatory cell infiltration, granulation tissue formation, and cartilage regeneration. In both DP-MSC groups, epithelial regeneration and granulation tissue increased, while epithelial degeneration and inflammation decreased. Collagen deposition increased in both treatment groups, whereas capillary density and cartilage regeneration were significantly higher only in the 1×10⁶ DP-MSC group.
CONCLUSION: DP-MSC application supports nasal septal wound healing and cartilage regeneration, and these effects were observed to increase in a dose-dependent manner. Considering the potential advantages of DP-MSCs compared to other MSC sources, they may have future clinical applicability with further supportive clinical studies.
Keywords: Septal Perforation, Dental Pulp, Mesenchymal Stem Cell, Wound Healing, Cartilage Regeneration