Pathological and ultrastructural changes in cultured cells induced by venom from the ectoparasitic wasp Nasonia vitripennis (Walker) (Hymenoptera Pteromalidae)


Rivers D. B., UÇKAN F., Ergin E., Keefer D. A.

JOURNAL OF INSECT PHYSIOLOGY, cilt.56, sa.12, ss.1935-1948, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 56 Sayı: 12
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.jinsphys.2010.08.019
  • Dergi Adı: JOURNAL OF INSECT PHYSIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1935-1948
  • Anahtar Kelimeler: Parasitoid, Venom, Programmed cell death, Apoptosis, Oncosis, TURIONELLAE L. HYMENOPTERA, SARCOPHAGA-BULLATA, FLESH FLY, PUPAL DIAPAUSE, HOST, APOPTOSIS, DEATH, ENVENOMATION, NECROSIS, DIPTERA
  • Kocaeli Üniversitesi Adresli: Evet

Özet

The ectoparasitic wasp Nasonia vitripennis produces a proteinaceous venom that induces death in fly hosts by non-paralytic mechanisms Previous in vitro assays have suggested that the primary cause of cell and tissue death is oncosis a non-programmed cell death (PCD) pathway characterized by cellular swelling and lysis However ultrastructural analyses of BTI-TN-5B1 cells exposed to LC(99) doses of wasp venom revealed cellular changes more consistent with apoptosis and/or non-apoptotic PCD than oncosis or necrosis By 3 h after incubation with venom susceptible cells displayed indentations in the nuclear membranes large nucleoli and extensive vacuolization throughout the cytoplasm In the vast majority of venom treated cells annexin V bound to the plasma membrane surface within 15 min after treatment a characteristic consistent with translocation of phosphatidylserine to the cell surface during the early stages of apoptosis Likewise mitochondrial transmembrane potential was depressed in cells within 15 min in venom-treated cells an event that occurred in the absence of mitochondrial swelling or rupturing of cristae Active caspase 3 was detected by fluorescent labeling in nearly all venom treated cells 3 h after exposure to venom and in turn the potent caspase 3 inhibitor Z-VAD-FMK attenuated the morphological changes elicited by wasp venom and afforded protection to BTI-TN-5B1-4 cells (C) 2010 Elsevier Ltd All rights reserved