Lessening the toxic effect of the methylisothiazolinone via vermicompost tea on Pisum sativum


ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2022
  • Doi Number: 10.1007/s11356-022-19396-4
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, IBZ Online, ABI/INFORM, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Chromosomal aberrations, Comet assay, DNA damage, Methylisothiazolinone, Pisum sativum, Vermicompost tea, DNA-DAMAGE, COMET ASSAY, PLANT-GROWTH, MYCORRHIZAL COLONIZATION, CELLS, SENESCENCE, BIOCIDES, DEGRADATION, KINETICS, WATERS
  • Kocaeli University Affiliated: Yes


Biocides, which are found in nature as persistent pollutants, pose a great danger to the ecosystem. Methylisothiazolinone (MIT), a widely used biocide, reaches plants by mixing with water and soil. Vermicompost tea (VCT), which strengthens the plant defence mechanism and increases its growth and development, is a liquid fertiliser consisting of the cooperation of worms with microbes. In the present study, after applying 0.4 g/L (EC50/2), 0.8 g/L (EC50), and 1.6 g/L (EC50 x 2) MIT concentrations without and with VCT on forage pea (Pisum sativum), root lengths, mitotic index data, chromosome and nuclei abnormalities, and DNA damage level were determined. When VCT applied and non-applied groups were compared, it was found that, especially in the VCT applied group, they cope with the stress conditions created by MIT. In addition, positive effects were observed in root lengths, mitotic index data, and amount of cell nuclei abnormalities. In line with other study results, VCT reduces cellular damage by regulating the normal life cycle disrupted in the cell due to mutagens using the curative-regulatory feature.