The effects of lps-induced preterm labor on learning and memory in adult WAG / Rij rats

Nuraydın A., Şahin D., Karadenizli Taşkın S., Şahin T., Ateş N.

SfN Connectome 2021, Washington, United States Of America, 11 January - 13 May 2021, pp.0-1

  • Publication Type: Conference Paper / Summary Text
  • City: Washington
  • Country: United States Of America
  • Page Numbers: pp.0-1
  • Kocaeli University Affiliated: Yes


OBJECTIVE: The risk of preterm birth increases in mothers diagnosed with gramnegative bacterial infection during pregnancy. This situation constitutes important risk factors in the formation of important neurological disorders such as epilepsy, white matter damage in the brain and cerebral palsy in premature babies. Lipopolysaccharide (LPS) is a commonly used agent to create a preterm delivery model in rats. In our study, it was planned to investigate the effects of LPS induced preterm model on neurobehavioral performance, learning and memory in the adult WAG/Rij rats. The aim of this study is to evaluate the effects of preterm birth on learning and memory in a pathological genetic background such as absence seizure activity.

METHODS AND METHODS: It was planned to be Group1(naive) n=8, Group2(SF) n=8, Group3(LPS) n=8.SF and/or Lipopolysaccharide(200µg/kg) were injected on the 15th and 16th day of pregnancy. At postnatal six month later, effects on locomotor activity, passive avoidance and morris-water-tank tests were investigated.

RESULTS: When locomotor activity was evaluated, there was no significant change in total activity between the groups. In the passive avoidance test, the retention time in the LPS induced group was shortened compared to the naive and SF control groups(p<0.05). In the Morris-water-tank test, in LPS induced group, there was a decrease in the correct squatting time according to the naive and SF control groups(p<0.05).

CONCLUSIONS: Idiopathic generalized epilepsy has a genetic basis, including absence epilepsy. WAG/Rij rats can be regarded as a valid genetic animal model of absence epilepsy with comorbidity of depression. Behavioral studies indicate that WAG/Rij rats exhibit depression-like symptomps. In WAG/Rij rats with genetically pathological background, premature birth induced by injection of LPS caused disruption in learning and memory tests in the adult period of the offspring. Further studies are needed to investigate the accompanying neurochemical and systemic changes in order to explain changes in learning and memory behavior.Keywords: Absence epilepsy, Passive avoidance, Water maze test