Cloning and expression of trehalose-6-phosphate synthase 1 from Rhizopus oryzae


Uyar E. O. , Yucel M., Hamamcı H.

JOURNAL OF BASIC MICROBIOLOGY, vol.56, pp.459-468, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 56
  • Publication Date: 2016
  • Doi Number: 10.1002/jobm.201500425
  • Title of Journal : JOURNAL OF BASIC MICROBIOLOGY
  • Page Numbers: pp.459-468

Abstract

Trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. In fungi, Trehalose-6-phosphate synthase 1 (TPS1) plays a key role in the biosynthesis of trehalose. In this study, a full-length cDNA from Rhizopus oryzae encoding TPS1 (designated as RoTPS1) was isolated. The RoTPS1 cDNA is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8kDa. The amino acid sequence of RoTPS1 has a relatively high homology with the TPS1s in several other filamentous fungi. RoTPS1 was cloned into Saccharomyces cerevisiae and secretively expressed.