Real-Time PCR analysis of af4 and dek genes expression in acute promyelocytic leukemia t(15;17) patients


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Savli H. , Sirma S., Nagy B., Aktan M., Dincol G., Salcioglu Z., ...More

EXPERIMENTAL AND MOLECULAR MEDICINE, vol.36, no.3, pp.279-282, 2004 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 3
  • Publication Date: 2004
  • Doi Number: 10.1038/emm.2004.38
  • Title of Journal : EXPERIMENTAL AND MOLECULAR MEDICINE
  • Page Numbers: pp.279-282

Abstract

Among several newly identified oncogenes, dek and af4 are attractive targets for researchers interested with leukemia. In this study quantitative Real-Time RT-PCR technique was used to define alterations in expression of dek and af4 genes associated with acute promyelocytic leukaemia (APL) t (15; 17). RNA samples obtained from bone marrow aspirates of fourteen APL patients, cDNA portions were labelled with Syber Green 1 dye and LightCycler analysis have been performed. Expression changes in patients were found not significant in comparison to healthy donors for af4 (P = 0.192) and dek (P = 0.089.5). We suggest that af4 gene may have a role in leukomogenesis restricted to lymphoblastic lineage; also further studies must carry on with a larger series of patients in order to understand the relationship between the dek gene and APL. Our study was the first attempt for analysing dek and af4 genes in APL t (15; 17) patients by quantitative Real-Time RT-PCR. This rapid and sensitive method could be used to screen these genes in different types of leukaemia.