Splenosis is a benign condition that frequently occurs after rupture of spleen following an abdominal trauma. We present a 28-year-old male patient who was referred to our hospital for laparoscopic cholecystectomy with a prior diagnosis of acute cholecystitis. The medical history revealed splenectomy following traumatic rupture of the spleen in 1983. The patient underwent a laparoscopic surgery for cholecystitis and a large mass lesion was detected in the liver. Although localization of the mass was thought to be atypical for splenosis, in order to rule out the possibility of splenosis, heat denatured red blood cell scintigraphy was performed. The study showed multiple focal areas of radiotracer uptake around the splenic bed and upper part of the liver parallel to the contour of the diaphragm which was consistent with the diagnosis of splenosis.