Akademik Veri Yönetim Sistemi
Perinatal Journal, cilt.32, sa.2, ss.166-172, 2024 (Scopus)
Objective: In this study, we examined the HOXA1 expression in the placentas of women diagnosed with fetal growth restriction (IUGR) by immunoexpression and in silico analysis. Methods: Placenta samples from 40 control (healthy) and 40 pregnant women diagnosed with IUGR were included in the study. The samples were fixed in zinc-formal and embedded in paraffin. Demographic information of the patients was recorded. Sections taken from paraffin blo-cks were analyzed by Hematoxylin-Eosin and HOXA1 immunostaining. The protein-protein interaction network of HOXA1 was constructed using the STRING database and analyzed with Cytoscape. The route description was made with the DAVID web tool. Results: In histopathological examination, intense fibrin accumulation, structural degeneration of placental components, congestion, dilatation and increased syncytial nodes were observed in the IUGR group compared to the control group. HOXA1 gene expression was significantly increased in the IUGR group. The HOXA1 PPI network contained 201 nodes and 3876 edges. MCODE analysis identified 8 modules, the highest scoring module was related to the “Systemic lupus erythematosus”, “Alcoholism” and “Neutrophil extracellular trap formation” pathways. Conclusion: With immunoexpression and in silico analysis, we showed HOXA1 is a player of immune pathways, tissue development, and placental regulation, suggesting potential research avenues in understanding IUGR mechanisms.