Akademik Veri Yönetim Sistemi
Acta Medica Nicomedia, cilt.8, sa.2, ss.229-232, 2025 (Hakemli Dergi)
ABSTRACT Objective: Isolation of RNA from serum samples has gained importance, especially in studies on the use of small RNA molecules such as miRNA as biomarkers. Selection of the optimal kit is critical for the accuracy of downstream processing. The aim of this study was to compare the performance of different commercial kits in terms of efficiency, RNA purity and contamination control during the isolation process. Method: Three different RNA isolation kits were used for 5 sheep serum samples: 1. miRNeasy Serum/Plasma Kit (Cat. No: 217184, Qiagen, USA), 2. Norgen Plasma/serum RNA purification kit (Cat. No: 55000, Norgen, Canada), 3. Nucleogene RNA isolation kit (Cat. No: NG044, Nucleogene, Turkey). The purity and intensity of the obtained RNAs were evaluated by measuring A260/280 ratios with a nanodrop spectrophotometer. Results: When the concentrations and A260/280 ratios obtained from the kits were evaluated by One Way Anova Test using GraphPad Prism (V10.4.0), it was observed that there was a statistically significant difference between the concentrations and A260/280 ratios of the 3 kits (p≤0.05 and p≤ 0.001). RNAs obtained from Norgene showed the lowest concentration and the lowest A260/280 ratiowhere as Nucleogene had the highest RNA concentration and A260/280 ratio of 2.0or higher among the three kits (p≤0.05). Conclusion: Among the kits used for serum RNA isolation, the Nucleogene kit stands out with the highest RNA yield and suitable A260/280 values in general. However, the Norgene and Qiagen kits may still be preferred under specific experimental conditions. Keywords: RNA isolation, serum, A260/280 ratio, commercial reagent kits