EFFECT OF EXOGENOUS GIBBERELLIC ACID AND PRESENCE OR ABSENCE OF TESTA ON THE EX VITRO SEEDLING GROWTH OF BAY LAUREL (LAURUS NOBILIS L.)


Cavusoglu A.

APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, vol.19, no.3, pp.2189-2200, 2021 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 3
  • Publication Date: 2021
  • Doi Number: 10.15666/aeer/1903_21892200
  • Journal Name: APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH
  • Journal Indexes: Science Citation Index Expanded, Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.2189-2200

Abstract

Seed germination, seedling growth and viability are the initial, critical steps and they are affected by various exogenous and endogenous factors including seed testa and plant growth regulators. This study investigated the effects of testa (seeds with or without testa) and gibberellic acid (GA 3 ; 0, 100, 500, 1000, 2000, 3000 and 4000 ppm) on seed germination, seedling growth parameters and seedling viability rate in bay laurel (Laurus nobilis L.). It has been definitely demonstrated that the seed testa extends seedling formation process at the final germination by nearly two times via delaying effects on the first germination time. For seed-with testa, all GA(3) concentrations were found to be similarly successful (between 83.3 and 88.3%) compared to control (68.3%) but in seed-without testa, high GA(3) concentrations (55.0% in 2000 ppm, 56.7% in 3000 ppm and 55.0% in 4000 ppm) showed greater negative effect on final germination than lower doses (80% in 100 ppm, 81.7% in 500 and 1000 ppm) and even control (63.3%). GA(3) concentrations showed no differences in most seedling parameters excluding root number in seeds with testa and maximum root length in seeds without testa. Despite the delaying effect of testa, seedling and viability capacity were higher than those of seeds without testa in high GA(3) concentrations. On the other hand, in control and lower doses of GA(3) (100, 200 and 500 ppm) gave similar results of these two parameters. All data confirmed that Laurus nobilis L. seed testa have effects on seed germination delaying but found more resistant to seedling capacity (83.09%) and seedling viability (94.39%) in average after acclimatization in which seed-without testa gave lower results with 67.63%; 78.24% respectively in the ex vitro studies.