2-methyl-2H-isothiazol-3-one (MIT), widely used in industrial and cosmetic products, is one of the significant representatives of the isothiazolinone group. Due to widespread application fields of MIT, it can be easily mixed with water and soil. Vermicompost (VCT), which has an enriching effect on the properties of the soil, provides nutrients to the plants and can transform the soil into a more livable form for plants. In this study, MIT was applied to the P. sativum, which has high nutrition content and is a significant food source for animals. Also, VCT was applied to decrease the stress stemming from the MIT chemical on the plant. In order to achieve this, the effective concentration (EC50) value of MIT was determined, and the EC50/2 and EC50x2 concentrations were used on the plant, and the biochemical effects (i.e., total protein, MDA and H2O2 amounts, SOD activity) at the end of the 48th and 72nd hours were examined. Then, the same chemical was applied with VCT, keeping all processes constant, and the same biochemical parameters were investigated. When the results were compared with the group treated with VCT, it was observed that there was a difference, especially in Total protein, MDA amounts, and SOD activity. When statistical data was compared with the control group, it was significantly different at the p < .05 level. This result implies that VCT can be a good solution for recyclings increased the amount of organic waste, and reducing the use of artificial fertilizers.