Plant regeneration and corm formation of saffron (Crocus sativus L.) in vitro


ÇAVUŞOĞLU A., Sulusoglu M., Erkal S.

RESEARCH JOURNAL OF BIOTECHNOLOGY, vol.8, no.12, pp.128-133, 2013 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 12
  • Publication Date: 2013
  • Journal Name: RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.128-133
  • Keywords: Crocus sativus, saffron, node culture, micropropagation, corm, STIGMA-LIKE-STRUCTURE, SOMATIC EMBRYOGENESIS, FLORAL ORGANS, INDUCTION, MICROPROPAGATION, EXPLANTS, GROWTH

Abstract

In vitro plant regeneration and daughter corm formation of saffron (Crocus sativus L.) from corm parts consisting of meristematic region via direct organogenesis were performed. In the first experiment, corm explants were treated with five different sterilization procedures in order to overcome contamination difficulties as being encountered in all the geophyte. In the second experiment on direct shoot regeneration and providing the foliation, sterile meristematic node-containing corm explants were cultured on Murashige and Skoog (MS) Medium or MS with several dose of BAP. According to the data MS+6 mg/l BAP had the best results in shoot initiation (96.7 %) and the foliation rate (93.3 %) while MS+1 mg/l BAP gave the least result in shoot initiation (16.7 %); MS+1 mg/l BAP and MS+10 mg/l BAP gave the least result in foliation from the initiated shoots in 90 days.