Plant regeneration and corm formation of saffron (Crocus sativus L.) in vitro

ÇAVUŞOĞLU A., Sulusoglu M., Erkal S.

RESEARCH JOURNAL OF BIOTECHNOLOGY, cilt.8, sa.12, ss.128-133, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 12
  • Basım Tarihi: 2013
  • Dergi Adı: RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.128-133
  • Anahtar Kelimeler: Crocus sativus, saffron, node culture, micropropagation, corm, STIGMA-LIKE-STRUCTURE, SOMATIC EMBRYOGENESIS, FLORAL ORGANS, INDUCTION, MICROPROPAGATION, EXPLANTS, GROWTH
  • Kocaeli Üniversitesi Adresli: Evet

Özet

In vitro plant regeneration and daughter corm formation of saffron (Crocus sativus L.) from corm parts consisting of meristematic region via direct organogenesis were performed. In the first experiment, corm explants were treated with five different sterilization procedures in order to overcome contamination difficulties as being encountered in all the geophyte. In the second experiment on direct shoot regeneration and providing the foliation, sterile meristematic node-containing corm explants were cultured on Murashige and Skoog (MS) Medium or MS with several dose of BAP. According to the data MS+6 mg/l BAP had the best results in shoot initiation (96.7 %) and the foliation rate (93.3 %) while MS+1 mg/l BAP gave the least result in shoot initiation (16.7 %); MS+1 mg/l BAP and MS+10 mg/l BAP gave the least result in foliation from the initiated shoots in 90 days.