The pain and behavioral traits of early Juvenile arthritis induced by collagen ii in rat models

Şahin N., Çelebi G., Bayrak Y. E., Tüfekçi G., Göçmez S. S., Sönmez H. E., ...Daha Fazla

The 31st European Paediatric Rheumatology Congress:, Gothenburg, İsveç, 11 - 14 Eylül 2024, cilt.22, ss.30-31, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Cilt numarası: 22
  • Basıldığı Şehir: Gothenburg
  • Basıldığı Ülke: İsveç
  • Sayfa Sayıları: ss.30-31
  • Kocaeli Üniversitesi Adresli: Evet

Özet

P237 The pain and behavioral traits of early Juvenile arthritis induced by collagen ii in rat models

Nihal Sahin1, Gülşen Çelebi2, Yunus E. Bayrak1, Gözde Tüfekçi2, Semil S. Göçmez2, Hafize E. Sönmez1, Tijen Utkan2
1Pediatric Rheumatology; 2Pharmacology, Kocaeli University, Kocaeli, Türkiye
Correspondence: Nihal Sahin

Pediatric Rheumatology 2024, 22(2): PReS24-ABS-1442


Introduction: Chronic rheumatic diseases, including juvenile idiopathic arthritis (JIA), can be associated with anxiety-depression-like behavior and cognitive decline. Collagen II-induced arthritis is the most commonly used rat model of rheumatoid arthritis. However, the characteristics of the collagen II-induced early juvenile arthritis model, which occurs in rats younger than five weeks before puberty, have not been clearly defined yet.

Objectives: The aim is to evaluate the relationship between JIA and pain, depressive behaviors, and cognitive changes by examining the characteristics of an early juvenile arthritis rat model.

Methods: Juvenile male Wistar albino rats were divided into three groups: control, arthritis, and sham. Arthritis was induced with collagen II emulsified in Freund's adjuvant in the arthritis group. Arthritis severity and pain were analyzed weekly. After six weeks, depression-like behavior and cognitive functions were evaluated.

Results: Initial joint diameters were similar across groups (p>0.05). Over weekly follow-ups, the arthritis group showed significantly increased joint diameters compared to others (p<0.05). The mechanical withdrawal threshold was consistently lower in the arthritis group for six weeks (p<0.05). The passive avoidance test had no significant difference (p>0.05). The arthritis group exhibited longer immobilization time in the modified forced swimming test (arthritis: 34.97±4.38 sec, control: 16.52±3.19 sec, sham: 21.35±3.79 sec; p=0.005).

Conclusion: Our investigation employing the early juvenile arthritis rat model has uncovered a dichotomy: in arthritis occurring at a remarkably young age, depression-like behaviors become conspicuous, while cognitive functions remain resilient.

Funding: This work has been supported by Kocaeli University Scientific Research Projects Coordination Unit under grant number TSA-2023-3336.

Date of birth:: 21.09.1987


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Disclosure of Interest

None Declared