Prunus laurocerasus L. is grown as a native fruit in the Eastern Black sea region of Turkey and has attracted attention because of promising fruit. It is mostly consumed as fresh fruit in local markets, but may also be dried and processed into jam or marmalade products. Rapid and clonal propagation of selected material is too important for the plantation of the commercial orchards. The aim of this investigation was to improve a protocol for micropropagation of Prunus laurocerasus L. in vitro. Shoot tips were sterilized with 20% commercial bleach (5% sodium hypoclorite) for 14 min and cultured in Murashige and Skoog Medium (MS) containing different concentrations of 6-benzylaminopurine (BAP) along with the control treatment. In some cases indole-3-butyric acid (IBA) was used in combination with BAP for shoot elongation, 2.0 mg/l BAP combined with 0.1 mg/l IBA gave the highest number of new shoots with a score of 6.13 and the mean length of shoots was 3.26 cm. Shoot length decreased with the increasing concentrations of BAP and IBA hormones. For rooting, explants of shoots 1-2 cm in length were transferred to different rooting treatments. The results revealed that the best rooting was obtained with 0.5 mg/l IBA in MS medium. The number and length of roots were 4.17 shoots/explants and 3.29 cm, respectively. Plantlets were successfully transferred to soil. A high survival rate was achieved with plants that were rooted in 0.5 mg/l IBA added culture medium (77.3%). The micropropagation protocol can be used for maintenance of the clonal propagation of P. laurocerasus L.