The Use of TurboID for Cell Surface Proteome Analysis in Breast Cancer

Şahin E. İ., Sarıhan M., Koçyiğit E., Kasap M., Akpınar G.

Uluslararası Proteomik Kongresi // 5. Ulusal Proteomik Kongresi, Ankara, Turkey, 13 October 2023 - 14 January 2024, pp.34

  • Publication Type: Conference Paper / Summary Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.34
  • Kocaeli University Affiliated: Yes

Abstract

The extracellular matrix (ECM) and cell junction (CJ) proteins provide structural and functional support to the cells and play several crucial roles e.g., cell adhesion, cell migration, cell signaling and com- munication under various processes. Elucidation of changes in ECM and CJ proteomes is important since they play crucial roles in the development, progression, and metastasis of various cancers, including breast cancer (BC). Therefore, we used a novel approach to study these proteomes of three BC cell lines to discover candidate biomarkers. For this, ECM and CJ proteins were labelled with TurboID, enriched via streptavidin and identified with nLC-MS/MS.