Ba-Alginate Microspheres for Protein Encapsulation by Aerosolization

Asri H., Liman G., Mert S.

JOURNAL OF APPLIED POLYMER SCIENCE, vol.142, no.14, 2025 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 142 Issue: 14
  • Publication Date: 2025
  • Doi Number: 10.1002/app.56692
  • Journal Name: JOURNAL OF APPLIED POLYMER SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aerospace Database, Applied Science & Technology Source, Biotechnology Research Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Keywords: biomaterials, biomedical applications, drug delivery systems
  • Kocaeli University Affiliated: Yes

Abstract

The encapsulation efficiency, release profiles, and kinetics of BSA from Ba-alginate microcapsules with the size of 150-215 mu m, prepared by aerosolization method starting from different BSA concentrations (w/v: 0.05%, 0.10%, 0.15%, and 0.30%), were investigated. The critical BSA w/v ratio was found to be 0.15%, and BSA release from both Ba- and Ca-alginate microcapsules, prepared for comparison, was continued up to 25 and 3 h, respectively. Korsmeyer-Peppas model was found to be valid for Ba-alginate microcapsules according to release kinetics analysis. BSA-loaded Ba-alginate microcapsules were more durable and had a longer release profile than Ca-alginate microcapsules. FTIR analysis of Ca-alginate and Ba-alginate microcapsules confirms the cross-linking of Ca2+ and Ba2+ ions with alginate and supports the presence of BSA in BSA-loaded Ba and Ca-alginate microcapsules. Moreover, the developed BSA-Ba-alginate microcapsules did not show swelling behavior at pH = 1.2 and remained intact in sodium citrate solution until 24 h. These results demonstrate that Ba-alginate microcapsules are resistant in the stomach environment and suitable for encapsulation of water-soluble molecules such as BSA.