Changes in cardiac cells due to ticagrelor and enoxaparin in a rat ischemia/reperfusion model.


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Fındık O., Baris Ö. , Yazir Y. , Yilmaz M. Y. , Rencber S. H. , Sarihan K. K. , ...More

Revista da Associacao Medica Brasileira (1992), vol.67, no.12, pp.1764-1770, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 12
  • Publication Date: 2021
  • Doi Number: 10.1590/1806-9282.20210477
  • Title of Journal : Revista da Associacao Medica Brasileira (1992)
  • Page Numbers: pp.1764-1770
  • Keywords: Ticagrelor, Enoxaparin, Ischemia, Rats, Reperfusion injury, ISCHEMIA, INJURY, CLOPIDOGREL, APOPTOSIS

Abstract

OBJECTIVE: Studies on ischemia/reperfusion injury remain the focus of interest. Ticagrelor and enoxaparin, which are antiaggregant and anticoagulant drugs developed for use in many cardiovascular pathologies, are still included in many ischemia/reperfusion studies. Remarkably, their new protective effects, especially with regard to ticagrelor, continue to be reported in the current literature. The aim of this study was to evaluate the beneficial effects of ticagrelor and enoxaparin pretreatments on the rat heart with histological and immunohistochemical markers in an ischemia/reperfusion model. METHODS: Wistar–albino rats (weighing 350–400 g) were divided into four groups as follows: Sham-Control (Group 1), ControlSaline+ischemia/reperfusion (Group 2), Ticagrelor+ischemia/reperfusion (Group 3), and Enoxaparin+ischemia/reperfusion (Group 4). The ischemia/reperfusion injury model was applied to Group 2, Group 3 and Group 4. Heart tissue sections were stained with hematoxylin and eosin for histological examinations. Caspase 3 immunostaining was evaluated to detect apoptosis in the heart tissue sections. RESULTS: Both pretreatments ameliorated the ischemic damage but especially tissue sections belonging to Group 3 were nearly similar to control levels. The results indicated that ischemia/reperfusion-induced myocardial damage was significantly increased in Group 2, whereas ticagrelor and enoxaparin pretreatments in Group 3 and Group 4 significantly decreased apoptotic scores and the histological appearance of the Group 3 close to the normal myocardium (p<0.001). CONCLUSION: As supported by histological findings in our study, ticagrelor and enoxaparin have protective properties for heart tissue in this ischemia/reperfusion injury model. KEYWORDS: Ticagrelor. Enoxaparin. Ischemia. Rats. Reperfusion injury.