Biodiesel, which can be produced from renewable sources such as vegetable oils or animal fats, is an alternative fuel for diesel engines. Sunflower and cottonseed oils are produced in Turkey dominantly. Using biodiesel in diesel engines will provide great benefits both environmentally and economically. Therefore, for biodiesel production, the availability of the oils produced in our country should be investigated and the studies on this subject should be increased. In this study, fatty acid methyl esters were produced from waste vegetable oil and edible vegetable oils. Some fuel properties of the methyl esters such as density, viscosity, total-free glycerol amounts, distillation temperatures, acid number, ester content, cetane index and pour point were measured and appropriateness of these properties for the biodiesel standards was investigated. In addition, some properties of glycerin, which is a by-product in ester production and used in many areas, were determined and compared with those of pure glycerin.