Enrichment and Identification of Bacterial Membrane Proteins by TurboID-Mediated Biotinylation

Koçyiğit E., Sarıhan M., Şahin E. İ., Akpınar G., Kasap M.

Uluslararası Proteomik Kongresi // 5. Ulusal Proteomik Kongresi, Ankara, Türkiye, 13 - 14 Ekim 2023, ss.59

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.59
  • Kocaeli Üniversitesi Adresli: Evet

Özet

Bacterial membrane proteins are pivotal players in cellular processes, exerting a profound impact on the overall function and survival of bacteria. These proteins are intricately associated with or embedded within the cell's membrane structure. However, the enrichment and identification of bacterial membrane proteins pose significant challenges due to factors like their inherent hydrophobic nature, relatively low abundance, and considerable heterogeneity. Despite all scientific developments, achieving comprehensive and ideal enrichment remains an ongoing endeavour.  In this study, our objective was to address these chal- lenges using TurboID, an evaluated biotin ligase, was used to label membrane proteins of E. coli (K12). This approach is designed to enhance the accuracy and efficiency of analysing these membrane proteins, shedding light on their roles within the bacterial cell and potentially advancing our understanding of bacterial physiology.