MXenes for CRISPR-based diagnostics: innovations and advancements

Amoozadeh M., Mohandesnezhad S., ZAREPOUR A., Khosravi A., Zarrabi A., Iravani S.

Microchemical Journal, cilt.218, 2025 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 218
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.microc.2025.115751
  • Dergi Adı: Microchemical Journal
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Food Science & Technology Abstracts, Index Islamicus, Veterinary Science Database
  • Anahtar Kelimeler: Biomedical applications, CRISPR-based diagnostics, Environmental applications, Food safety, MXenes
  • Kocaeli Üniversitesi Adresli: Evet

Özet

The incorporation of MXenes into CRISPR-based diagnostics marks a transformative advancement in molecular testing across environmental monitoring, biomedical diagnostics, and food safety. MXenes, a class of two-dimensional materials characterized by their exceptional electrical conductivity, hydrophilicity, and customizable surface properties, significantly enhance the sensitivity and specificity of CRISPR assays. Recent developments demonstrate that MXene composites improve the stability and performance of CRISPR systems, facilitating rapid and accurate detection of pathogens and genetic markers. These capabilities support applications such as analyzing environmental DNA (eDNA), point-of-care testing for infectious diseases, and identifying contaminants in food products. Despite these advancements, challenges such as sample processing, stability, the necessity for nucleic acid amplification, and regulatory barriers complicate practical implementations. This review explores the recent advancements of MXene-assisted CRISPR diagnostics, examines the challenges hindering their broader application, and discusses future perspectives for improving detection capabilities and integrating innovative technologies to enhance diagnostic efficacy.