Akademik Veri Yönetim Sistemi
3rd International Paris Congress on Medical & Health Sciences, Paris, Fransa, 1 - 03 Temmuz 2024, ss.53-54
Abstract Introduction and Purpose: According to world cancer research statistics, breast cancer is second most common type of cancer regardless of gender, ranks first among newly diagnosed cancers among women with a rate of 25% and one in eight women in the world develops breast cancer. Many environmental and genetic factors play a role in the etiology of cancer. The circadian clock is responsible for the coordination of the 24-hour oscillation of the environmental time cycle in almost all tissues in the body. MicroRNAs (miRNAs) are 18-21 nucleotide long, single-stranded, noncoding RNA molecules that alter gene expression at the post-transcriptional stage. Based on this information, we hypothesized that, the expression of the CLOCK gene conserved miRNA hsa-miR217 in healthy and tumor tissue samples from 20 patients diagnosed with breast cancer was compared by real time PCR. Materials and Methods: From 20 patients diagnosed with breast cancer, healthy and tumor tissue samples were obtained from the same individuals during total or partial mastectomy surgery and stored at -80°C until RNA isolation. Total RNA isolation, cDNA synthesis revealed and gene expression were compared by RT- PCR. For gene expression estimation, 2-ΔΔCt values were used and the differences between the tissues were compared with T-test using SPSS package program. Results: hsa-miR-217 expression between healthy and tumor tissues was significantly different (p<0.05). Discussion and Conclusion: The present study demonstrated that hsa-miR-217 expression is significantly different in tumor and healthy breast tissue. There are studies showing the association of circadian rhythm dysregulation and hsa-miR-217 with cancer in different cancer types separately, but this is the first study to identify the CLOCK gene and miR-217 in breast cancer. Further analysis and further studies are needed to determine the potential of the gene and miR-217 as breast cancer markers, and this study is a preliminary investigation. Key Words: Breast cancer, miRNA, Circadian rhythm, Clock gene, Real Time PCR