Akademik Veri Yönetim Sistemi
49th FEBS Congress: Bridging Continents to Advance Life Science, İstanbul, Türkiye, 5 - 09 Temmuz 2025, cilt.15, ss.41, (Özet Bildiri)
Evaluation of the relationship between serum and pleural fluid lipid raft
protein levels with plasma inflammatory biomarkers
O. Demirtas *I,II,III,IV, F. Hunc *III, A. ElicoraI, S. Argun BarisII, H.F. SezerI, M.O. DillioglugilIII
IDepartment of Thoracic Surgery, School of Medicine, Kocaeli University, Izmit, Kocaeli, Türkiye, IzmitKocaeli,
Türkiye, IIDepartment of Chest Diseases, School of
Medicine, Kocaeli University, Izmit, Kocaeli, Türkiye, IzmitKocaeli,
Türkiye, IIIDepartment of Biochemistry, School of Medicine, Kocaeli University, Izmit, Kocaeli,
Türkiye, IzmitKocaeli,
Türkiye, IVDepartment of Medical Biochemistry, Institute of Health Sciences, Kocaeli University, Izmit, Kocaeli, Türkiye, IzmitKocaeli,
Türkiye
Raftlin is lipid raft related protein, plays distinct roles in cellular functions, including protein trafficiking, apoptosis, proliferation, adhesion, and cell signaling. In this study,
we aim to explore relationship between pleural fluid and serum raftlin proteins with plasma inflammatory biomarkers.
This prospective casecontrol
study was approved from local ethical committee of Kocaeli University (GOKAEK2020/
10.15). At total, 80 subjects’ biological
samples were collected, yet 52 of the samples were included in analysis due to lack of missing data. Pleural fluid and blood samples of the pleural effusion patients were
collected concomitantly. Pleural effusion cytology were used as initial step for differential diagnosis of malign etiology. Routine biochemical analysis for each samples
were performed to define characteristics of pleural effusion. Inflammatory indices of hemogram was employed to test association with serum and pleural fluid raftlin
protein levels. Remaining samples were stored at 80
°C until further ELISA analysis of the raftlins.
Raftlins didn’t differ from the patients having malignnonmalign
etiology for pleural effusion. Moreover, plasma inflammatory biomarkers were found to be uncorrelated
with both serum and pleural raftlin levels. Besides, it was found that there was no difference in terms of raftlins whether effusion was exudative or transudative. Pleural
fluid and serum samples were found to be similar for raftlin proteins.
For the first time in literature, we foud that, serum and pleural raftlin concentrations were highly compatible with each other. Whereas current body of knowledge for
lipid raft proteins point out the association with inflammation, both pleural and serum raftlin protein levels didn’t correlated with 12 hematological inflammatory indices.
These inconsistent findings could be resulted from the factors causing pleural effusion. Thus, further research is needed to determine role of lipid raft proteins in pleural
effusion.
* The authors marked with an asterisk equally contributed to the work.