The aim of this study was to determine the effect of spirituality on the levels of hope in women undergoing infertility treatment. This cross-sectional study was completed with the participation of 150 women undergoing IVF treatment at a university hospital in Kocaeli. In this study the women's spiritual well-being levels were high, and their hope levels were above the average score. Significant correlations were found between hope, spirituality, educational status, and age. Since attempts to boost hope will increase the chance of success in treatment, it is thought that factors affecting the level of hope should be determined and addressed. It is also thought that health professionals can apply hope therapy to women and take initiatives to provide spiritual support.