Akademik Veri Yönetim Sistemi
5th International Molecular Immunology and Immunogenetics Congress, İzmir, Türkiye, 20 - 22 Ekim 2022, ss.1
Highly induced inflammation, like it was observed in the CoVid-19 infections, could have devastating effects on thetissue health and its function. In many studies, mesenchymal stem cells (MSC) have been shown to functionappropriately to suppress this adverse interaction of T-lymphocytes with other cells by paracrine effect. However,the targeting of MSC to T-lymphocytes and the sublevel expression of cell adhesion receptors are consideredamong the important limitation of MSC-based therapies.
In this study, it was aimed to enhance the interaction of Wharton’s Jelly (WJ)-MSC with T-lymphocytes bytransferring the anti-CD2 (LFA2) antibody in a conjugated-complex with fatty acids to become a cell membraneelement, and to investigate the effect of this interaction on phytohemagglutin-stimulated T lymphocytes. Aftercoating the anti-CD2 molecule to CD2
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MSC, the cell viability by WST, the cell counting by flow cytometer and cell-to-cell interactions by photomicrographs were evaluated.
The amount of fatty acid complex was first optimized due to its cytotoxicity effect in cell culture. Following thecoating with conjugated-complex, the coating efficiency of WJ-MSC was found to be as 49,5% by flow cytometer.Under the microscope, the binding of T-lymphocytes with WJ-MSC was observed. The flow cytometer analysis alsoconfirmed this interaction: the level of MSC bound with CD3+ T-lymphocytes was low, but the cell numbers werefound to be higher in the anti-CD2 coated group, where the cell viability estimated by WST was higher.
By this experimental approach, the improvement of the effectiveness of WJ-MSC-T-lymphocyte cell interaction wasobserved. Thus, the immune modulatory properties of MSCs were improved significantly. The T-lymphocytestargeting of MSC via anti-CD2 in vitro showed that this approach might be applicable in the treatment ofautoimmune diseases.