Cloning and expression of trehalose-6-phosphate synthase 1 from Rhizopus oryzae


Uyar E. O. , Yucel M., Hamamcı H.

JOURNAL OF BASIC MICROBIOLOGY, cilt.56, ss.459-468, 2016 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 56
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1002/jobm.201500425
  • Dergi Adı: JOURNAL OF BASIC MICROBIOLOGY
  • Sayfa Sayıları: ss.459-468

Özet

Trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. In fungi, Trehalose-6-phosphate synthase 1 (TPS1) plays a key role in the biosynthesis of trehalose. In this study, a full-length cDNA from Rhizopus oryzae encoding TPS1 (designated as RoTPS1) was isolated. The RoTPS1 cDNA is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8kDa. The amino acid sequence of RoTPS1 has a relatively high homology with the TPS1s in several other filamentous fungi. RoTPS1 was cloned into Saccharomyces cerevisiae and secretively expressed.