Akademik Veri Yönetim Sistemi
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, cilt.26, ss.330-334, 2021 (SCI-Expanded)
Objectives : Antibiotic therapy for Pseudomonas infections is becoming increasingly difficult. In this study, the transposons from two multidrug-resistant (MDR) clinical Pseudomonas strains containing related transposons responsible for giving rise to resistance determinants were characterised. Methods : Two MDR clinical Pseudomonas isolates were obtained from a medical facility in Cyprus. The strains were identified as Pseudomonas putida C54 and Pseudomonas aeruginosa C69. DNA was extracted from both strains and was sequenced. Transposons were identified, annotated and compared with DNA sequences in GenBank. Results : Two related nested transposons, here named Tn 6608 (from P. putida C54) and Tn 6609 (from P. aeruginosa C69), were characterised. The transposons are built on an ancestral Tn 1403 base element (here named Tn 1403A ) that contains only the transposition module ( tnpA and tnpR ) and the associated cargo gene module ( orfA, orfB, orfC and orfD ) flanked by a 38-bp inverted repeat. The nested transposons identified in this study have evolved via acquisition of multiple transposons, adding multiple resistance genes to an ancestral transposon that originally lacked any resistance determinants. Conclusion : Transposons related to Tn 6608 and Tn 6609 have evolved and are globally disseminated. Of particular interest is that most of these nested transposons are located within the same site in a genomic island, providing alternative avenues for dissemination. (c) 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )