Three-Phase Partitioning as a Rapid and Easy Method for the Purification and Recovery of Catalase from Sweet Potato Tubers (Solanum tuberosum)

DUMAN Y., KAYA E.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, cilt.170, sa.5, ss.1119-1126, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 170 Sayı: 5
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1007/s12010-013-0260-9
  • Dergi Adı: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1119-1126
  • Kocaeli Üniversitesi Adresli: Evet

Özet

Three-phase partitioning (TPP) was used to purify and recover catalase from potato crude extract. The method consists of ammonium sulfate saturation, t-butanol addition, and adjustment of pH, respectively. The best catalase recovery (262 %) and 14.1-fold purification were seen in the interfacial phase in the presence of 40 % (w/v) ammonium sulfate saturation with 1.0:1.0 crude extract/t-butanol ratio (v/v) at pH 7 in a single step. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the enzyme showed comparatively purification and protein molecular weight was nearly found to be 56 kDa. This study shows that TPP is a simple, economical, and quick method for the recovering of catalase and can be used for the purification process.