Visual-Based Contact Detection for Automated Zebrafish Larva Heart Microinjection


Zhang G., Tong M., Qian C., Zhuang S., Wang C., Yu X., ...More

IEEE TRANSACTIONS ON AUTOMATION SCIENCE AND ENGINEERING, vol.18, no.4, pp.1803-1813, 2021 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 4
  • Publication Date: 2021
  • Doi Number: 10.1109/tase.2020.3019782
  • Title of Journal : IEEE TRANSACTIONS ON AUTOMATION SCIENCE AND ENGINEERING
  • Page Numbers: pp.1803-1813
  • Keywords: Microinjection, Heart, Skin, Image edge detection, Calibration, Cameras, Active contours, Coordinate transformation, curve evolution, edge detection, microinjection, zebrafish larva, CELLS, MODEL

Abstract

This article presents an automated strategy to touch the injection site on zebrafish larva skin with the injection pipette tip accurately in the presence of water-depth variation, which is a crucial problem to automate zebrafish larva microinjection. The presented method consists of two parts: adaptive coordinate transformation and curve evolution for edge detection. In the first part, the impact of refraction is taken into consideration. An adaptive calibration method is developed, which enables the coordinate transformation matrix to adapt to the changing water depth. In the second part, the abovementioned calibration result is used to keep the injection pipette tip descending along the desired route. A curve-evolution-based edge detection algorithm is introduced to detect the deformation of larva skin caused by contact with the injection pipette tip. Experimental results demonstrate that high accuracy and success rates are achieved. The effect of uncertainties caused by water-depth variation and the skill requirement in manual manipulation are eliminated. The proposed contact detection strategy can be extended to microinjection for other organisms.