Protective effect of oltipraz in testicular ischaemia/reperfusion injury: An experimental study


Can O., Canat L., ERALDEMİR F. C. , ACAR E. , Yildirim F., Sonmez K., ...More

ANDROLOGIA, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2021
  • Doi Number: 10.1111/and.14245
  • Title of Journal : ANDROLOGIA
  • Keywords: ischaemia, oltipraz, oxidative stress, reperfusion injury, testicular torsion, ISCHEMIA-REPERFUSION INJURY, ISCHEMIA/REPERFUSION INJURY, OXIDATIVE STRESS, N-ACETYLCYSTEINE, NUCLEAR FACTOR, FACTOR-2 NRF2, TORSION, ANTIOXIDANT, PRETREATMENT, INHIBITION

Abstract

Testicular torsion is an emergency urological disease, and the treatment is immediate surgery. Despite emergency surgery, testicular damage may occur due to reperfusion. Therefore, a medical treatment to prevent this damage may be a rational idea. We aimed to evaluate the protective effect of oltipraz in testicular ischaemia/reperfusion damage. Twenty-eight Wistar-Albino rats were randomly divided into four groups. In ischaemia/reperfusion group, testicular torsion was executed, and orchiectomy was done 4 hr after detorsion with no treatment. Second group performed torsion; intraperitoneal 50 mg/kg oltipraz was applied 30 min before detorsion, and orchiectomy was performed 4 hr after detorsion. Third group applied torsion; intraperitoneal 150 mg/kg oltipraz was applied 30 min before detorsion, and orchiectomy was performed 4 hr after detorsion. Last one was the sham group. We evaluated tissue malondialdehyde (MDA), transforming growth factor-beta 1 (TGF-beta 1), superoxide dismutase (SOD), reduced glutathione (GSH) and Johnsen testicular biopsy score. There was a significant decrease in TGF-beta 1, GSH and MDA values in oltipraz treatment groups compared with ischaemia/reperfusion group. Oltipraz treatment has significant protective effect in testicular ischaemia/reperfusion damage. However, more clinical studies are needed to demonstrate appropriate dose and its effects.