Characterization of polyphenol oxidase from fennel (Foeniculum vulgare Mill.) seeds as a promising source

YÜZÜGÜLLÜ KARAKUŞ Y., Yildirim B., ACEMİ A.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.170, ss.261-271, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 170
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.ijbiomac.2020.12.147
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.261-271
  • Anahtar Kelimeler: Fennel seeds, Polyphenol oxidase, Enzyme purification, Enzyme characterization, Enzyme inhibition, Organic solvents
  • Kocaeli Üniversitesi Adresli: Evet

Özet

Fennel seeds were recognized as a promising polyphenol oxidase (PPO) source upon investigating some edible green plants (carob, jujube, coriander, fennel, and licorice). The fennel PPO enzyme was purified by three-phase partitioning and biochemically characterized in detail for the first time. The purification fold and activity recovery values were determined as 20-fold and 120%, respectively. Its molecular weight was 27.8 kDa. The temperature for the selected substrates (catechol, 4-tert-butylcatechol, 4-methylcatechol, and pyrogallol) was 30 degrees C, while the optimum pH value varied from 5.0 to 7.0 depending on the substrate. The k(cat)/Km values exhibited that the enzyme presented the best activity towards catechol among the substrates used. Sodium metabisulfite, ascorbic acid, benzoic acid, L-cysteine, thiourea, beta-mercaptoethanol, and glutathione prominently inhibited PPO activity. A remarkable decrease in PPO activity was observed at elevated concentrations of organic solvents, but in cases of the solvents with polarity indexes >= 5.1, the residual activity maintained more than 75% of its original activity up to 10% (v/v). Consequently, the current study suggested that fennel seeds could be used in various industrial sectors to produce low-cost polyphenol oxidase enzymes with an agricultural origin. (C) 2020 Elsevier B.V. All rights reserved.