Suppression of generalized seizures activity by intrathalamic 2-chloroadenosine application

Ates N. , Ilbay G. , Sahin D.

EXPERIMENTAL BIOLOGY AND MEDICINE, cilt.230, ss.501-505, 2005 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 230 Konu: 7
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1177/153537020523000709
  • Sayfa Sayıları: ss.501-505


In the present study, we investigated the effects of micro-injecting 2-chloroadenosine (2-CADO; an adenosine receptor agonist) into the thalamus alone and with theophylline (a nonspecific adenosine receptor antagonist) pretreatment on pentylenetetrazol (PTZ)-induced tonic-clonic seizures in male Wistar albino rats. Following intrathalamic 2-CADO injection alone or theophylline pretreatment, 50 mg kg(-1) PTZ was given ip after 1 and 24 hrs. The duration of epileptic seizure activity was recorded by cortical electroencephalogram (EEG), and seizure severity was behaviorally scored. Intrathalamic 2-CADO administration induced significant decreases in both seizure duration and seizure severity scores at 1 and 24 hrs, but the effects were more abundant on the seizures induced after 24 hrs. On the other hand, pretreatment with theophylline prevented the inhibitor effect of 2-CADO on seizure activity and increased both seizure duration and seizure scores. Present results suggest that the activation of adenosine receptors in the thalamus may represent another anticonvulsant/modulatory site of adenosine action during the course of the PTZ-induced generalized tonic-clonic seizures and provide additional data for the involvement of the adenosinergic system in the generalized seizures model.