GENETIC DIVERSITY AND CONSERVATION STRATEGIES OF SOME LILIUM CANDIDUM L. POPULATION IN TURKEY


ÖZEN F. , AKA G. E. , AKSOY Ö.

BANGLADESH JOURNAL OF BOTANY, cilt.45, ss.133-141, 2016 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 45 Konu: 1
  • Basım Tarihi: 2016
  • Dergi Adı: BANGLADESH JOURNAL OF BOTANY
  • Sayfa Sayısı: ss.133-141

Özet

Lilium candidum L. is an economically important plant. Due to overcollection from nature and destruction of its habitats, it is included in the "vulnerable" category" of danger. Genetic diversity of five different populations of L. candidum, growing in Marmara region of Turkey naturally was investigated. These populations were compared with Kusadasi population that was selected for comparasion group in Aegean region of Turkey. It is aimed to developed conservation strategies in order to protect the species. RAPD-PCR analysis was performed with genomic DNA extracted from the leaves of each replicate of samples. Twenty-one primers were screened, but only six primers gave clear, reproducible banding patterns and selected were further analysis. Jaccard's genetic distances were calculated and dendogram was generated using the UPGMA algorithm. The dendogram obtained were classified into two main groups and three subgroups. Genetic distance and the polymorphic band ratio was determined as 0.0464 - 0.3619 and 74.47%, respectively between the populations. The populations that had the lowest polymorphism ratio were, Nusret (19.15%) and abla (19.15%). The populations that had the highest polymorphism ratio was found as Kecidere (25.53%). According to our findings the population of Kecidere and then all of the other populations in Marmara region of Turkey should be conserved in situ. In addition conservation of this species in botanical gardens, gene banks and agrosystems will support in situ management.