Torque transducer is mechanical device that converts an energy form to another energy form. Torque transducers are calibrated by using machines such as dead weight calibration machines and reference torque transducer systems. Torque transducers are at risk of being affected by various environmental conditions such as temperature and humidity, and deviating from expected measurement results due to strain gauges on them. These factors should be kept under control, if this is not possible, contribution of ambient temperature and air humidity effects on measurement results should be taken into consideration. In this study, measurements were made according to DIN 51309 directive, and effect of other parameter changes on measurement uncertainty, while temperature and humidity kept constant in turn, was investigated. In this study, the goal was to reduce measurement uncertainty of calibration by determining the optimum temperature and humidity values in test environment. As a result of this study, the optimum values are obtained. The lowest measurement uncertainty was found at 21 degrees C and 50 % RH in this experimental study. In a test environment where optimum environmental conditions are obtained as a result of this study, the torque transducers will have the opportunity to work with maximum precision.