Arginine mediated photodynamic therapy with silicon(IV) phthalocyanine photosensitizers

Balçık Erçin P., Ekineker G., Salik N., Aydogdu B., Yagci T., Göksel Şahin M.

PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, cilt.43, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.pdpdt.2023.103667
  • Dergi Adı: PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, MEDLINE
  • Anahtar Kelimeler: Arginine, DNA damage, Photodynamic therapy, Silicon(IV) phthalocyanine, Targeted agent, The cytotoxic effect
  • Kocaeli Üniversitesi Adresli: Evet

Özet

In the current study, we synthesized a new SiPc derivative conjugated with arginine at the axial positions, for a novel phthalocyanine-based photosensitizer for photodynamic therapy (PDT) applications in cancer cells. Axially-di-arginine substituted new silicon(IV) phthalocyanine photosensitizer (PS-5a) has been thoroughly researched for its anti-cancer properties. Various spectroscopic techniques were used to characterize this conjugate, including 1H NMR, 13C NMR, FT-IR, UV-vis, and MS spectral data. The in vitro PDT activities of the conjugate on cancer cells were tested through its cytotoxic, clonogenic, apoptotic effects on, and its capacity to induce DNA damage, and the disruption of mitochondrial membrane potential in cancer cell lines (liver; HuH-7, cervix; HeLa and breast; MCF7). Cancer cells exposed to the light illumination following uptake of the PS-5a as a photosensitizer revealed DNA breakage and collapsed mitochondrial membrane potential. The results of the present investigation demonstrate that PS-5a has a significant photo-cytotoxic effect on cancer cells. So, axiallydi-arginine substituted silicon(IV) phthalocyanine could be an effective PDT agent for PDT treatment.