The objective of this study was to discover the incidence of Chlamydia trachomatis in women with urethral syndrome. A total of 102 patients included in this study were divided into two groups: group 1 comprised 63 women suffering from painful urination and frequency, with no organisms seen on Gram stain or isolated in routine Urine culture; group 2 consisted of 39 women with no complaints. Chlamydia antigen was found to be positive in 24 patient samples (38%) in group 1 and in four patient samples (11%) in group 2. The difference between the two groups was statistically significant (p=0.0435). There was also a weak correlation between Chlamydia antigens and finding more than 10 polymorphonuclear leucocytes in the Gram stains (r=0.02364).