Akademik Veri Yönetim Sistemi
V. International Agricultural, Biological & Life Science Conference, Edirne, Türkiye, 18 - 20 Eylül 2023, ss.740-744
Polyphenol oxidases (PPOs), which have been the subject of numerous studies in recent years and have attracted the attention of researchers, are enzymes that catalyze the oxidation of phenolic compounds. Although PPOs are generally found in plants, they are also found in many microorganisms and fungi. Therefore, PPOs have a wide distribution. PPOs are used in many fields, especially in medicine, cosmetics, and industry. The aim of this study was to investigate PPO by selecting fruits with high lycopene content. For this purpose, 9 different fruits (watermelon, bell pepper, fig, pomegranate, blood orange, grapefruit, cranberry, rosehip, potency pomegranate) were extracted in vitro using 13 different extraction methods (10 mM ascorbic acid in extraction buffer, 2 mM phenylmethylsulfonyl fluoride, 1% polyvinylpolypyrrolidone (w/v), 1% Triton X-100 (v/v), pH 7.0) and enzyme activity was measured spectrophotometrically.