In this work, one-bit transform (1BT) based motion estimation method is applied to HEVC encoder which is the most recent video coding standard. The most significant portion of the computational load of the HEVC originates from the motion estimation. Thus, many approaches are presented in literature in order to reduce this computational load. 1BT based ME approach denotes input image frames in low-bit depth form so that it is not only suitable for hardware implementation but also reduces computational load of whole process since it uses EX-OR based matching criterion. In this work, 1BT based ME is integrated into HEVC encoder and its performance is assessed. Experimental results show that the 1BT based ME approach has a neglectable performance loss compared conventional HEVC motion estimation approach.