Protection of rat pancreatic islet function and viability by coculture with rat bone marrow-derived mesenchymal stem cells

Karaoz E., Genç Z., Demırcan P. C., Aksoy A., Duruksu G.

CELL DEATH & DISEASE, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2010
  • Doi Number: 10.1038/cddis.2010.14
  • Journal Name: CELL DEATH & DISEASE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: pancreatic islet, rat bone marrow, mesenchymal stem cells, indirect coculture, antiapoptotic genes, ALLOGRAFT SURVIVAL, INSULIN-SECRETION, NITRIC-OXIDE, TRANSPLANTATION, ACTIVATION, PROMOTES, CYTOPROTECTION, FIBROBLASTS, INHIBITION, APOPTOSIS
  • Kocaeli University Affiliated: Yes

Abstract

The maintenance of viable and functional islets is critical in successful pancreatic islet transplantation from cadaveric sources. During the isolation procedure, islets are exposed to a number of insults including ischemia, oxidative stress and cytokine injury that cause a reduction in the recovered viable islet mass. A novel approach was designed in which streptozotocin (STZ)-damaged rat pancreatic islets (rPIs) were indirectly cocultured with rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) to maintain survival of the cultured rPIs. The results indicated that islets cocultured with rBM-MSCs secreted an increased level of insulin after 14 days, whereas non-cocultured islets gradually deteriorated and cell death occurred. The cocultivation of rBM-MSCs with islets and STZ-damaged islets showed the expression of IL6 and transforming growth factor-beta 1 in the culture medium, besides the expression of the antiapoptotic genes (Mapkapk2, Tnip1 and Bcl3), implying the cytoprotective, anti-inflammatory and antiapoptotic effects of rBM-SCs through paracrine actions. Cell Death and Disease (2010) 1, e36; doi: 10.1038/cddis.2010.14; published online 22 April 2010