Protection of rat pancreatic islet function and viability by coculture with rat bone marrow-derived mesenchymal stem cells

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Karaoz E., Genç Z., Demırcan P. C., Aksoy A., Duruksu G.

CELL DEATH & DISEASE, cilt.1, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1038/cddis.2010.14
  • Dergi Adı: CELL DEATH & DISEASE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Anahtar Kelimeler: pancreatic islet, rat bone marrow, mesenchymal stem cells, indirect coculture, antiapoptotic genes, ALLOGRAFT SURVIVAL, INSULIN-SECRETION, NITRIC-OXIDE, TRANSPLANTATION, ACTIVATION, PROMOTES, CYTOPROTECTION, FIBROBLASTS, INHIBITION, APOPTOSIS
  • Kocaeli Üniversitesi Adresli: Evet

Özet

The maintenance of viable and functional islets is critical in successful pancreatic islet transplantation from cadaveric sources. During the isolation procedure, islets are exposed to a number of insults including ischemia, oxidative stress and cytokine injury that cause a reduction in the recovered viable islet mass. A novel approach was designed in which streptozotocin (STZ)-damaged rat pancreatic islets (rPIs) were indirectly cocultured with rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) to maintain survival of the cultured rPIs. The results indicated that islets cocultured with rBM-MSCs secreted an increased level of insulin after 14 days, whereas non-cocultured islets gradually deteriorated and cell death occurred. The cocultivation of rBM-MSCs with islets and STZ-damaged islets showed the expression of IL6 and transforming growth factor-beta 1 in the culture medium, besides the expression of the antiapoptotic genes (Mapkapk2, Tnip1 and Bcl3), implying the cytoprotective, anti-inflammatory and antiapoptotic effects of rBM-SCs through paracrine actions. Cell Death and Disease (2010) 1, e36; doi: 10.1038/cddis.2010.14; published online 22 April 2010