Akademik Veri Yönetim Sistemi
V. in vitro Diyagnostik Sempozyumu, İzmir, Türkiye, 19 - 21 Şubat 2020, cilt.45, sa.1, ss.20, (Özet Bildiri)
OP-13
[DETECTION of POLYCHLORINATED BIPHENYLS IN
NEWBORNS BY GC-MS METHOD: INVESTIGATION of
THYROID FUNCTIONS]
[YENİDOĞANLARDA GC-MS YÖNTEMI İLE POLİKLORLU
BİFENİLLLERİN TESPİTİ: TİROD FONKSİYONLARININ
İNCELENMESİ]
Fatih HUNÇ1, Berkay YEŞİLDAĞLI2, Tuğba AYAZ2, Şule KOÇ1,
Ezginur KOCAKIR1, Meltem DİLLİOĞLUGİL1, Demet
ARSLANBAŞ2, Mihriban CİVAN2
1Kocaeli University, Faculty of Medicine, Department of Medical
Biochemistry, Kocaeli, Turkey
2Kocaeli University, Faculty of Engineering, Department of
Environmental Engineering, Kocaeli, Turkey
Anahtar Kelimeler:
Abstract
Objectives:
HIF1-α ekspresyon seviyelerindeki farklılıkları analiz
etmek için Western Blot yöntemi kullanıldı. Hücrenin epitelyal ve
mezenkimal fenotipinin karakterizasyonu için epitelyal belirteç olarak
E-cadherinin ve mezenşimal belirteç olarak da N-cadherinin
belirlenmesi için fluorosan immünositokimya yöntemi kullanılmıştır.
Persistent organic pollutants (POPs; polychlorinated
biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs),
polybrominated diphenyl ethers (PBDE)s and organochlorine
pesticides (OCPs)) are synthetic, lipophilic, organic chemicals that
remain in the environment for a long time. Environmental exposure
via food chain, which magnify its harmful effects is a major health
problem. PCBs congeners (Polychlorinated biphenyls; C12H10
−xClx) are one of the groups of POPs.
In this study, it is aimed to measure PCBs congeners with TSH
levels in neonatal serum according to its importance for neonates.
As we know, pregnant women, embryos and infants, may be more
vulnerable because of effects on thyroid hormone balance, and
central nervous system development.
Six infants whose cord blood samples were
obtained, included in the study. Besides this condition, infants, whom
sampling time for TSH was before 24 hours after birth and one baby
whose sampling time was after 96 hours after birth were excluded.
All instrumental analysis, included of total number of 14
polychlorinated biphenyls’ measurement was operated with Agilent
6890 GC-ECD detector. The extraction and clean-up columns were
operated as described in Standard Reference Material (1958) of US
National Institute of Standards & Technology, with minor
modifications. PCBs analysis were conducted in cord blood samples
of neonates due to observe intrauterine exposure and TSH analysis
were carried out in KOU Hospital central laboratory serums.
Biological samples preparation, acquisition of plasma and serum
samples were also performed at Department of Medical
Biochemistry. Samples were stored with appropriate standards in
glass vials. PCBs measurement was carried out by Department of
Environmental Engineering.
TSH and total lipid levels respectively measured with DxI 800 UniCel
(Beckman Coulter®) and AU 5800 Series (Beckman Coulter®) auto-
analyzers. For total lipid determination, respectively total cholesterol
analyzed by enzymatic colorimetric methods and then calculation was
performed via the formulation obtained from the literature.
Although the quantitative analysis of PCBs was performed
over 14 congeners, the pollutant concentration in the samples remained
under the limit of detection (LOD) of the method is shown as "-".
For PCBs, significant biochemical changes associated with exposure
expressed in the literature are reported at> 400 ng / g lipid levels. In our
study, 1100 ng / g level was determined for PCB-20 in a single sample.
However, no relation was found between TSH and PCB levels. Among
14 PCB congeners analyzed in this study, detected only markedly
higher 9 of them were highlighted in the Table. POPs have certain
affinity to lipids and the data here presented in both mL serum and
shown in ng per mg lipid.
In our study, as in some similar studies, no relationship
was found between TSH and polychlorinated biphenyls.
PCBs were one of the highly produced and used products in last
decade and regulative arrangements showing the results by finding
lesser concentration in human serum. Neonatal PCB levels are
important to monitor in order to showcase the possible detrimental
effects on thyroid on behalf of intrauterin exposure.
Keywords: Neonates, TSH, PCBs, persistent organic pollutant