Lipase catalysis: an environmentally friendly production for polyol esters (biolubricant) from microalgae oil

KUTLUK T., Kutluk B.

ENVIRONMENTAL TECHNOLOGY, vol.44, no.27, pp.4099-4112, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 27
  • Publication Date: 2023
  • Doi Number: 10.1080/09593330.2022.2079999
  • Journal Name: ENVIRONMENTAL TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, EMBASE, Environment Index, Geobase, Greenfile, INSPEC, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, DIALNET, Civil Engineering Abstracts
  • Page Numbers: pp.4099-4112
  • Keywords: Biolubricant, Chlorella protothecoides, esterification, lipase, microalgae oil, response surface methodology, FATTY-ACID ESTERS, IMMOBILIZED LIPASE, ENZYMATIC TRANSESTERIFICATION, NOVOZYM 435, CASTOR-OIL, OPTIMIZATION, ULTRASOUND, IMPROVE, WASTE, ESTERIFICATION
  • Kocaeli University Affiliated: Yes

Abstract

This study aims to investigate the optimized lipase-catalysed esterification reaction from novel feedstock microalgae (Chlorella protothecoides) oil-free fatty acids (FFAs) for biolubricant (Trimethylolpropane (TMP)-triesters) synthesis. FFAs were obtained from microalgae oil by enzymatic hydrolysis. Response surface methodology (RSM) with the central composite design was performed to investigate the effect of experimental factors (lipase amount, TMP/FFAs molar ratio, reaction temperature) on the FFAs conversion and also investigated to resolve the optimum design points. After the experimental studies, the highest FFAs conversion of 93% with 92% triester and 8% mono, di esters contents were found when the lipase amount was 5.5%. TMP/FFAs molar ratio was 0.33 and the reaction temperature value was 60 degrees C. The model fitted with the experimental values with R-2 = 0.97. It was also supported by gas chromatography and FTIR analyses that the product obtained was a lubricant.