Determination of the Optimum Culture Medium for In vitro Development of Festuca rubra ssp. rubra L.


Acemi A.

IV. International Eurasian Agriculture and Natural Sciences Congress, Konya, Türkiye, 30 - 31 Ekim 2020, ss.27

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Konya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.27
  • Kocaeli Üniversitesi Adresli: Evet

Özet

The current study aimed to determine the optimum culture medium composition for a better in vitro development of Festuca rubra ssp. rubra L. cv. Maxima 1. Developmental parameters evaluated based on the morphometric data were used to determine the optimum medium among the De Greef & Jacobs (DG), Linsmaier & Skoog (LS), Murashige & Skoog (MS), and Schenk & Hildebrandt (SH) media tested. SH medium gave the highest germination rate (78%), while the lowest germination rate (73%) was calculated from MS medium. However, the germination data remained statistically the same. The statistically same leaf numbers were recorded from DG (2.05) and SH (2.03) media. However, the seeds sown to MS, and LS media gave fewer leaves (1.63-1.38). The mean leaf lengths had a similar statistical trend with the mean leaf numbers. SH and DG media gave 9.73 cm and 9.14 cm mean leaf lengths, respectively. MS and DG media gave the highest root induction ratio with the mean root numbers 2.81 and 2.75, respectively. However, the SH medium gave the lowest mean root number (2.29). A similar trend in root elongation data was found among the media tested. The longest roots (4.26 cm) were measured from the plants grown in MS medium, while LS medium gave the shortest roots (2.45 cm). In conclusion, DG or SH media should be preferred over the other medium to achieve a better foliar growth of F. rubra ssp. rubra. However, MS and DG media were found more efficient in rhizogenesis in the same species.

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