Matlab-SIMULINK based real time temperature control of oven using different kinds of auto-tuning PID (Proportional-Integral-Derivative) methods is presented in this paper. Ziegler Nichols Step Response Method (P,PI), Relay Tuning Method (P,PI) and Integral Square Time Error (ISTE) disturbance criterion (PI) method are used to control temperature of the experiment set. Controller parameters are defined by simulations of auto-tuning methods. These parameters are used to implement the real time control of the oven designed as an experiment set. Three set point changes are realized during the experiments and the responses of the oven to these methods are observed. Finally the results are discussed to decide which controller is the best for set point changes.