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IMASCON 2021 International Marmara Sciences Congress, Kocaeli, Turkey, 21 May 2021, pp.75

  • Publication Type: Conference Paper / Summary Text
  • City: Kocaeli
  • Country: Turkey
  • Page Numbers: pp.75
  • Kocaeli University Affiliated: Yes


Fungicides are used to protect agricultural products and to minimize the damage which caused by fungal pathogens, These chemical products used in high amounts in our country, besides being used in the fight against pests, also harm non-target organisms like earthworms, soil bacteria etc. No study was found on the toxic effects of maneb fungicide on Eisenia fetida (red California worm). E. fetida is a widely used model organism and is preferred especially in studies examining the effects of environmental pollutants. The aim of this study was to determine the median lethal concentration (LC50) value and to investigate the effects of different concentrations of maneb on E. fetida. Six different concentrations of maneb (500, 600, 700, 800, 900 and 1000 mg/kg dry soil) and control group (no treatment) were used. The experiment procedure was carried out acoording to OECD guidelines. Three replicates were performed for each concentration. The final weights of the worms, morphological abnormalities and the mortality percentage were recorded on the 14th day.The statistical analysis of data was performed using SPSS 26.0 statistical software (SPSS Inc., Chicago, IL, USA). Probit analysis was used to determine LC50 value. Data for growth-inhibition level was subjected to one-way analysis of variance (ANOVA) using Fisher’s least significant difference test for contrast of the differences among treatment means. The data were displayed as means ± standard deviation (SD), and pvalues less than 0.05 were considered statistically significant. LC50 value was 872 mg/kg. It was observed that maneb caused an increase in growth inhibition parallel to increasing concentration. While the average weight of the worms in the control group increased by 27 %, the average weight decreased by 40 % at a concentration of 1000 mg / kg. All of the worms were examined and no observed any morphological abnormalities except just one from the highest concentration.It was determined that the worms exposed to the maneb were negatively affected in the artificial soil. Based on this, it was revealed that maneb fungicide may cause toxic effects on non-target organisms. Alternative biological methods may be preferred in pest control.