Akademik Veri Yönetim Sistemi
IMASCON 2021 International Marmara Sciences Congress, Kocaeli, Türkiye, 21 Mayıs 2021, ss.75
Fungicides are used to protect agricultural products and to minimize the damage which caused by fungal
pathogens, These chemical products used in high amounts in our country, besides being used in the fight
against pests, also harm non-target organisms like earthworms, soil bacteria etc. No study was found on the
toxic effects of maneb fungicide on Eisenia fetida (red California worm). E. fetida is a widely used model
organism and is preferred especially in studies examining the effects of environmental pollutants. The aim
of this study was to determine the median lethal concentration (LC50) value and to investigate the effects of
different concentrations of maneb on E. fetida. Six different concentrations of maneb (500, 600, 700, 800,
900 and 1000 mg/kg dry soil) and control group (no treatment) were used. The experiment procedure was
carried out acoording to OECD guidelines. Three replicates were performed for each concentration. The final
weights of the worms, morphological abnormalities and the mortality percentage were recorded on the 14th
day.The statistical analysis of data was performed using SPSS 26.0 statistical software (SPSS Inc., Chicago,
IL, USA). Probit analysis was used to determine LC50 value. Data for growth-inhibition level was subjected
to one-way analysis of variance (ANOVA) using Fisher’s least significant difference test for contrast of the
differences among treatment means. The data were displayed as means ± standard deviation (SD), and pvalues less than 0.05 were considered statistically significant. LC50 value was 872 mg/kg. It was observed
that maneb caused an increase in growth inhibition parallel to increasing concentration. While the average
weight of the worms in the control group increased by 27 %, the average weight decreased by 40 % at a
concentration of 1000 mg / kg. All of the worms were examined and no observed any morphological
abnormalities except just one from the highest concentration.It was determined that the worms exposed to
the maneb were negatively affected in the artificial soil. Based on this, it was revealed that maneb fungicide
may cause toxic effects on non-target organisms. Alternative biological methods may be preferred in pest
control.