Synergistic Effects of Salicylic Acid and Calcium Oxide Nanoparticles on Physiological and Biochemical Quality of Fresh-Cut Lettuce During Cold Storage


Güvenaltın Y., Demirel M., Samet H., Kasım M. U., Kasım R.

Horticulturae, cilt.11, sa.12, ss.1-22, 2025 (Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 12
  • Basım Tarihi: 2025
  • Doi Numarası: 10.3390/horticulturae11121438
  • Dergi Adı: Horticulturae
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.1-22
  • Kocaeli Üniversitesi Adresli: Evet

Özet

Fresh-cut lettuce rapidly deteriorates during storage, and developing safe strategies to

maintain its quality is critical. This study evaluated the effects of 2 mM salicylic acid (SA),

100 mg L−1 calcium oxide nanoparticles (CaNP), and their combination (CaNP + SA), and

an untreated control on the physiological and biochemical quality of fresh-cut Lactuca sativaL. var. crispa stored for 18 days at 5 ± 1 ◦C. Treatments were assessed through measurements

of total soluble phenolics (TSP), membrane stability index (MSI), chlorophyll components

(Chl a, Chl b, Total Chl), chlorophyll stability index (CSI), relative chlorophyll content

(SPAD), weight loss (WL), cutting resistance (CR), and color parameters. Both SA and

CaNP + SA significantly delayed senescence and oxidative degradation compared with

the control. The CaNP + SA combination showed the most comprehensive protection,

exhibiting the lowest WL (3.91%) and the highest MSI and CSI values, indicating enhanced

membrane integrity and pigment stability. SA alone maintained elevated TSP, SPAD,

and chlorophyll contents, supporting its antioxidative and metabolic regulatory roles.

Correlation analysis revealed strong positive relationships among MSI, CSI, SPAD, and

chlorophyll variables, while WL and CR were negatively associated with these traits. PCA

clearly separated treatments, with SA and CaNP + SA clustering with stability-related

parameters. Overall, CaNP + SA effectively preserved membrane function, pigment

structure, and water balance, representing a promising postharvest strategy for extending

the shelf life of fresh-cut lettuce.