Akademik Veri Yönetim Sistemi
15th International Hippocrates Congress on Medical and Health Sciences15-16 Mart 2024, TURKEY, Elazığ, Türkiye, 28 - 29 Haziran 2024, cilt.1, ss.1-2, (Özet Bildiri)
Measuring the Effectiveness of Mesenchymal Stem Cell-Supported Resveratrol Cultured in the Lung Cancer Microenvironment in Combined Treatment With Hyperbaric Oxygen Application: Preliminary Study Data Abstract Most lung cancers are epithelial cell carcinomas, with their progression and metastasis influenced by genetic factors and the immune system's interaction with the tumor microenvironment (TME). Mesenchymal stem cells (MSCs), which are multipotent and can self-renew, contribute to the tissues of multicellular organisms. The TME plays a crucial role in cancer angiogenesis, metastasis, and drug resistance. Resveratrol (RSV), a natural polyphenol in various plants, exhibits anti-inflammatory, antioxidant, anti-apoptotic, cardioprotective, cytoprotective, and anti-cancer properties. Hyperbaricoxygen-therapy (HBOT), involving 100% oxygen at high pressure, promotes cellular and vascular proliferation and negatively impacts tumors. Antioxidants induce necrotic or apoptotic cell death in cancer cells. Objective: To apply bone-marrow-derived-mesenchymal-stem-cell (BM-MSC) supported RSV and HBOT to TME of lung cancer cells for therapeutic purposes, to reduce oxidative stress, to stop tumour progression, to increase apoptosis and thus to prevent metastasis formation. Methods: After characterisation of BM-MSCs by flow cytometry, cytotoxicity of RSV on MSCs was measured by CCK8 viability assay. TME was established and therapeutic doses of RSV was administered at 24 and 48 hours. Effect of RSV on proliferation in TME was evaluated by Annexin V-PI assay. HBOT application was performed. Viability rate was determined with CCK-8. Results: Characterisation of BM-MSCs cells was performed by flow cytometry analysis. As a result of different concentration and time studies, it was determined by CCK-8 analysis that RSV was effective at 100µm at 48 hours. TME was established, effective dose of RSV was given and apoptosis experiments were performed with Annexin V-PI. It was shown by CCK-8 analysis that HBOT application at +1atm pressure decreased effect of HBOT on viability of cancer cells. Data obtained by using RSV in HBOT and RSV in TME revealed that studies should be carried out at molecular level. This study was supported by Kocaeli University BAP Coordination Unit. Project code: TYL-2023-3473. Keywords: Stem cell, lung cancer, HBOT, Resveratrol, apoptosis, proliferation, tumour microenvironment