A commercial lipase Resinase (R) HT (Aspergillus oryzae) efficiency on triglycerides transesterification and process optimization

KUTLUK T., Kutluk B. G.

SUSTAINABLE CHEMISTRY AND PHARMACY, cilt.30, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.scp.2022.100862
  • Dergi Adı: SUSTAINABLE CHEMISTRY AND PHARMACY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Biofuel, Resinase HT, Response surface methodology, Transesterification, STERYL ESTERASE, OIL, SPECTROSCOPY
  • Kocaeli Üniversitesi Adresli: Evet

Özet

In this study, the efficiency of Resinase HT enzyme on the transesterification reaction was investi-gated. Response surface methodology (RSM) was applied for optimization. After the experimen-tal studies the lowest fatty acid methyl ester (FAME) conversion was found as 71% when the en-zyme content was 15%(g% w/w), oil/methanol molar ratio was 0.17 and the reaction tempera-ture value was 45 degrees C. According to the design response, 70.83% was observed in the same condi-tions. The highest FAME conversion of 97% was found when the enzyme content was 5% (g% w/ w), oil/methanol molar ratio was 0.25 and the reaction temperature value was 35 degrees C. The experi-mental run gave the FAME conversion of 96.80% at the same reaction conditions. The model fit-ted with the experimental values with R2 = 0.98.