Study of alkaline phosphatase from human hydatidiform mole
Pakistan Journal of Medical Sciences, cilt.24, sa.3, ss.471-474, 2008 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 24 Sayı: 3
- Basım Tarihi: 2008
- Dergi Adı: Pakistan Journal of Medical Sciences
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.471-474
- Anahtar Kelimeler: Alkaline phosphatase, Hydatidiform mole, Ion exchange
- Kocaeli Üniversitesi Adresli: Evet
Özet
Objective: Study was performed on purification of alkaline phosphatase from Hydatiform mole. Methodology: The sample of mole Hydatidiform subjects from Taleghani Hospital, Tehran, Iran was studied. An Alkaline phosphatase from Human Hydatidiform mole was purified by a protocol involving solubilization using precipitation by butanol, acetone, Ammonium sulphate, Sephadex G200, Ion exchange chromatography and preparative electrophoresis. Results: The enzyme was purified 800-fold to apparent homogeneity. This enzyme has 5.2% carbohyrate content. The optimum Temperature and pH were 40 centigrade degree and 10.4, respectively. Conclusion: Human Hydatidiform mole alkaline phosphatase is a novel alkaline phosphatase.