Ciprofloxacin and Purple Carrot Extract-Loaded Bilayer Nanofibrous Wound Dressings: A Multifunctional Platform for Controlled Release and Antibacterial Wound Care
POLYMERS, cilt.18, sa.20, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 18 Sayı: 20
- Basım Tarihi: 2026
- Doi Numarası: 10.3390/polym18202479
- Dergi Adı: POLYMERS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, Compendex, INSPEC, Academic Search Ultimate (EBSCO), Engineering Source (EBSCO), Materials Science & Engineering Collection (ProQuest), Technology Collection (ProQuest)
- Kocaeli Üniversitesi Adresli: Evet
Özet
To effectively manage bacterial infections and support tissue regeneration, developing multifunctional, biocompatible, and stable wound coverings is critical. Here, bilayer nanofiber wound dressings were developed via electrospinning, comprising a ciprofloxacin-loaded (CIP) polyvinyl alcohol (PVA) layer and a purple carrot extract-loaded (PCrt) polyvinylpyrrolidone (PVP) layer, and cross-linked with glutaraldehyde (GLA). Compared to the PVA/CIP-PVP control, which exhibited restricted swelling (similar to 2.65%) and limited CIP release (8.51%), incorporating hydrophilic PCrt (7% w/w) enhanced macroscopic swelling to 707.14% and provided diffusion-controlled antioxidant capacity (12.86% via DPPH). Kinetic modeling identified Quasi-Fickian diffusion, while the Peppas-Sahlin model mathematically proved that severe chain entanglement and hydrogen bonding locked the matrix (negative relaxation constant), suppressing proportional release efficiency to similar to 22.8%. Biologically, the PCrt-free control showed GLA-induced basal cytotoxicity (66.31% viability) and 0% antibacterial activity due to strict drug confinement. However, PCrt addition restored L929 fibroblast viability to safe levels (75-77%) via antioxidant phenolic protection. Crucially, PCrt incorporation effectively mobilized residual GLA and phenolic compounds, elevating bactericidal efficacy to 100% against both Escherichia coli and Staphylococcus aureus. Although these bilayer nanofibers show strong synergistic bactericidal effects, crosslinking strategies on the green concept should be developed to eliminate GLA-induced toxicity and ensure wound management in safe clinical conditions.