Atıf İçin Kopyala
İNCİ D., AYDIN R., Zorlu Y.
JOURNAL OF MOLECULAR STRUCTURE, cilt.1225, 2021 (SCI-Expanded)
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Yayın Türü:
Makale / Tam Makale
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Cilt numarası:
1225
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Basım Tarihi:
2021
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Doi Numarası:
10.1016/j.molstruc.2020.129099
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Dergi Adı:
JOURNAL OF MOLECULAR STRUCTURE
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Derginin Tarandığı İndeksler:
Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, INSPEC
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Kocaeli Üniversitesi Adresli:
Evet
Özet
Two water soluble copper(II) mono-anionic glutamatecomplexes-[Cu(phen)(glu)(H2O)]NO3H2O (1) and [Cu(py-phen)(glu)(H2O)]NO3H2O (2) (phen: 1,10-phenanthroline, py-phen: pyrazino[2,3-f][1,10]phenanthroline, glu: glutamic acid), have been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complex with biomacromolecules {calf thymus DNA (CT-DNA) and bovine serum albumin (BSA)} has been investigated by electronic absorption and fluorescence spectroscopy methods. The experimental outcomes displays that the complexes 1 and 2 bind to CT-DNA by means of a moderate intercalation mode. Furthermore, the fluorescence quenching mechanism between BSA and the complexes 1 and 2 is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (AG, AH, AS) of BSA + the complexes 1 and 2 systems were determined at different temperatures. The binding distance between BSA and the complexes 1 and 2 was calculated according to Forster non-radiation energy transfer theory (FRET). The effect of the complexes 1 and 2 on the conformation of BSA was also examined using synchronous, two dimensional (2D) and three dimensional (3D) fluorescence spectroscopy. Radical scavenging activity of the complexes 1 and 2 was determined in terms of IC50, using the DPPH and H2O2 method. In biomacromolecules interactions and radical activity studies, the complexes 1 and 2 were found to give good results. (C) 2020 Elsevier B.V. All rights reserved.