Investigation on water soluble copper(II) mono-anionic glutamate complexes with planar aromatic ligands: synthesis, crystal structures, biomacromolecular interactions and radical scavenging activities


İNCİ D. , AYDIN R., Zorlu Y.

JOURNAL OF MOLECULAR STRUCTURE, vol.1225, 2021 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 1225
  • Publication Date: 2021
  • Doi Number: 10.1016/j.molstruc.2020.129099
  • Title of Journal : JOURNAL OF MOLECULAR STRUCTURE

Abstract

Two water soluble copper(II) mono-anionic glutamatecomplexes-[Cu(phen)(glu)(H2O)]NO3H2O (1) and [Cu(py-phen)(glu)(H2O)]NO3H2O (2) (phen: 1,10-phenanthroline, py-phen: pyrazino[2,3-f][1,10]phenanthroline, glu: glutamic acid), have been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complex with biomacromolecules {calf thymus DNA (CT-DNA) and bovine serum albumin (BSA)} has been investigated by electronic absorption and fluorescence spectroscopy methods. The experimental outcomes displays that the complexes 1 and 2 bind to CT-DNA by means of a moderate intercalation mode. Furthermore, the fluorescence quenching mechanism between BSA and the complexes 1 and 2 is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (AG, AH, AS) of BSA + the complexes 1 and 2 systems were determined at different temperatures. The binding distance between BSA and the complexes 1 and 2 was calculated according to Forster non-radiation energy transfer theory (FRET). The effect of the complexes 1 and 2 on the conformation of BSA was also examined using synchronous, two dimensional (2D) and three dimensional (3D) fluorescence spectroscopy. Radical scavenging activity of the complexes 1 and 2 was determined in terms of IC50, using the DPPH and H2O2 method. In biomacromolecules interactions and radical activity studies, the complexes 1 and 2 were found to give good results. (C) 2020 Elsevier B.V. All rights reserved.